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Author Lerche, A.; Bisgaard, H.; Christensen, J.D.; Venge, P.; Dahl, R.; Sondergaard, J. url  openurl
  Title Lactoferrin, myeloperoxidase, lysozyme and eosinophil cationic protein in exudate in delayed type hypersensitivity Type Journal Article
  Year (down) 1988 Publication Allergy Abbreviated Journal Allergy  
  Volume 43 Issue 2 Pages 139-145  
  Keywords Adult; Aged; Biopsy; Blood Proteins/*analysis/pharmacokinetics; Cell Cycle/drug effects; Dermatitis, Contact; Eosinophil Granule Proteins; Exudates and Transudates/*analysis/cytology; Female; Humans; Hypersensitivity, Delayed/*metabolism/physiopathology; Lactoferrin/*analysis/pharmacokinetics; Lactoglobulins/*analysis; Male; Middle Aged; Neutrophils/cytology/metabolism/physiopathology; Nickel/pharmacology; Peroxidase/*analysis/pharmacokinetics; *Ribonucleases; Skin/cytology; Skin Window Technique  
  Abstract A skin window technique was used to study the morphology of leucocytes in upper dermis and exudate during nickel challenge in patients with contact allergy to nickel. Contact allergic patients and healthy volunteers tested with a skin widow without addition of nickel to the chamber medium served as controls. The morphology of the leucocytes in dermis was studied in biopsies taken 8, 24, or 48 h after skin window application, and in a parallel test the morphology of the exudate was examined by sequential collection of the chamber medium during a 48 h period. The infiltrate in dermis of contact allergic patients with nickel challenge in the chamber medium showed a time-dependent increase of mononuclear cells, eosinophils and basophils and a concomitant decrease of polymorphonuclear granulocytes, characteristic of a combined specific and unspecific inflammation. The morphology of the exudate in contact allergic patients exposed to nickel showed a dominance of polymorphonuclear granulocytes throughout the study period, while mononuclear cells, eosinophils and basophils were detected at a much lower quantity and with a considerable delay. Further, we studied the kinetics of the leucocyte granule proteins: lactoferrin, myeloperoxidase, lysozyme and eosinophil cationic protein in exudate fluid in a parallel test. A significant higher flux was found for all during the second day of allergen exposure compared to contact allergic patients without allergen challenge as well as normal volunteers. The increased protein fluxes were not accompanied by an increased flux of polymorphonuclear granulocytes in the exudate.(ABSTRACT TRUNCATED AT 250 WORDS)  
  Address Dept. of Dermatology, Bispebjerg Hospital, Copenhagen, Denmark  
  Corporate Author Thesis  
  Impact Factor 06,028 First Author Lerche, A. Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Senior Author Sondergaard, J.  
  ISSN 0105-4538 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:2834974 Approved no  
  Call Number Serial 307  
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